Estrogen-related receptor γ is an in vivo receptor of bisphenol A.
Identifieur interne : 003513 ( PubMed/Curation ); précédent : 003512; suivant : 003514Estrogen-related receptor γ is an in vivo receptor of bisphenol A.
Auteurs : Marie Tohmé [France] ; Sophie M. Prud'Homme [France] ; Abdel Boulahtouf [France] ; Eric Samarut [France] ; Frédéric Brunet [France] ; Laure Bernard [France] ; William Bourguet ; Yann Gibert [Australie] ; Patrick Balaguer [France] ; Vincent Laudet [France]Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Danio zébré, Membrane des statoconies, Récepteurs des oestrogènes.
- pharmacologie : Composés benzhydryliques, Phénols.
- Animaux, Liaison aux protéines, Membrane des statoconies.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Receptors, Estrogen.
- chemical , pharmacology : Benzhydryl Compounds, Phenols.
- drug effects : Otolithic Membrane, Protein Binding.
- metabolism : Otolithic Membrane, Zebrafish.
- Animals.
Abstract
Bisphenol A (BPA) is an endocrine disruptor that displays estrogenic activity. Several reports suggest that BPA may have estrogen receptor-independent effects. In zebrafish, 50 μM BPA exposure induces otic vesicle abnormalities, including otolith aggregation. The purpose of this study was to test if BPA action was mediated in vivo during zebrafish development by the orphan nuclear estrogen related receptor (ERR) γ. Combining pharmacological and functional approaches, we demonstrate that the zebrafish ERRγ mediates BPA-induced malformations in otoliths. Using different bisphenol derivatives, we show that different compounds can induce a similar otolith phenotype than BPA and that the binding affinity of these derivatives to the zebrafish ERRγ correlates with their ability to induce otolith malformations. Morpholino knockdown of ERRγ function suppresses the BPA effect on otoliths whereas overexpression of ERRγ led to a BPA-like otolith phenotype. Moreover, a subphenotypical dose of BPA (1 μM) combined with ERRγ overexpression led to a full-dose (50 μM) BPA otolith phenotype. We therefore conclude that ERRγ mediates the otic vesicle phenotype generated by BPA. Our results suggest that the range of pathways perturbed by this compound and its potential harmful effect are larger than expected.-Tohmé, M., Prud'homme, S. M., Boulahtouf, A., Samarut, E., Brunet, F., Bernard, L., Bourguet, W., Gibert, Y., Balaguer, P., Laudet, V. Estrogen-related receptor γ is an in vivo receptor of bisphenol A.
DOI: 10.1096/fj.13-240465
PubMed: 24744145
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William Bourguet<affiliation><nlm:affiliation>INSERM U1054, Centre de Biochimie Structurale, Montpellier, CNRS UMR5048, Universités Montpellier 1 and 2, Montpellier, France; and.</nlm:affiliation><wicri:noCountry code="subField">France; and</wicri:noCountry></affiliation>Le document en format XML
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Prud'Homme</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon</wicri:regionArea></affiliation></author><author><name sortKey="Boulahtouf, Abdel" sort="Boulahtouf, Abdel" uniqKey="Boulahtouf A" first="Abdel" last="Boulahtouf">Abdel Boulahtouf</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier</wicri:regionArea></affiliation></author><author><name sortKey="Samarut, Eric" sort="Samarut, Eric" uniqKey="Samarut E" first="Eric" last="Samarut">Eric Samarut</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon</wicri:regionArea></affiliation></author><author><name sortKey="Brunet, Frederic" sort="Brunet, Frederic" uniqKey="Brunet F" first="Frédéric" last="Brunet">Frédéric Brunet</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon</wicri:regionArea></affiliation></author><author><name sortKey="Bernard, Laure" sort="Bernard, Laure" uniqKey="Bernard L" first="Laure" last="Bernard">Laure Bernard</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon</wicri:regionArea></affiliation></author><author><name sortKey="Bourguet, William" sort="Bourguet, William" uniqKey="Bourguet W" first="William" last="Bourguet">William Bourguet</name><affiliation><nlm:affiliation>INSERM U1054, Centre de Biochimie Structurale, Montpellier, CNRS UMR5048, Universités Montpellier 1 and 2, Montpellier, France; and.</nlm:affiliation><wicri:noCountry code="subField">France; and</wicri:noCountry></affiliation></author><author><name sortKey="Gibert, Yann" sort="Gibert, Yann" uniqKey="Gibert Y" first="Yann" last="Gibert">Yann Gibert</name><affiliation wicri:level="1"><nlm:affiliation>Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin School of Medicine, Waurn Ponds, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin School of Medicine, Waurn Ponds, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Balaguer, Patrick" sort="Balaguer, Patrick" uniqKey="Balaguer P" first="Patrick" last="Balaguer">Patrick Balaguer</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier</wicri:regionArea></affiliation></author><author><name sortKey="Laudet, Vincent" sort="Laudet, Vincent" uniqKey="Laudet V" first="Vincent" last="Laudet">Vincent Laudet</name><affiliation wicri:level="1"><nlm:affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France; vincent.laudet@ens-lyon.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon</wicri:regionArea></affiliation></author></analytic><series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title><idno type="eISSN">1530-6860</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Benzhydryl Compounds (pharmacology)</term><term>Otolithic Membrane (drug effects)</term><term>Otolithic Membrane (metabolism)</term><term>Phenols (pharmacology)</term><term>Protein Binding (drug effects)</term><term>Receptors, Estrogen (metabolism)</term><term>Zebrafish (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Composés benzhydryliques (pharmacologie)</term><term>Danio zébré (métabolisme)</term><term>Liaison aux protéines ()</term><term>Membrane des statoconies ()</term><term>Membrane des statoconies (métabolisme)</term><term>Phénols (pharmacologie)</term><term>Récepteurs des oestrogènes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Receptors, Estrogen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Benzhydryl Compounds</term><term>Phenols</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Otolithic Membrane</term><term>Protein Binding</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Otolithic Membrane</term><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Danio zébré</term><term>Membrane des statoconies</term><term>Récepteurs des oestrogènes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Composés benzhydryliques</term><term>Phénols</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Liaison aux protéines</term><term>Membrane des statoconies</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bisphenol A (BPA) is an endocrine disruptor that displays estrogenic activity. Several reports suggest that BPA may have estrogen receptor-independent effects. In zebrafish, 50 μM BPA exposure induces otic vesicle abnormalities, including otolith aggregation. The purpose of this study was to test if BPA action was mediated in vivo during zebrafish development by the orphan nuclear estrogen related receptor (ERR) γ. Combining pharmacological and functional approaches, we demonstrate that the zebrafish ERRγ mediates BPA-induced malformations in otoliths. Using different bisphenol derivatives, we show that different compounds can induce a similar otolith phenotype than BPA and that the binding affinity of these derivatives to the zebrafish ERRγ correlates with their ability to induce otolith malformations. Morpholino knockdown of ERRγ function suppresses the BPA effect on otoliths whereas overexpression of ERRγ led to a BPA-like otolith phenotype. Moreover, a subphenotypical dose of BPA (1 μM) combined with ERRγ overexpression led to a full-dose (50 μM) BPA otolith phenotype. We therefore conclude that ERRγ mediates the otic vesicle phenotype generated by BPA. Our results suggest that the range of pathways perturbed by this compound and its potential harmful effect are larger than expected.-Tohmé, M., Prud'homme, S. M., Boulahtouf, A., Samarut, E., Brunet, F., Bernard, L., Bourguet, W., Gibert, Y., Balaguer, P., Laudet, V. Estrogen-related receptor γ is an in vivo receptor of bisphenol A.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24744145</PMID><DateCreated><Year>2014</Year><Month>07</Month><Day>29</Day></DateCreated><DateCompleted><Year>2014</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2014</Year><Month>07</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1530-6860</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>7</Issue><PubDate><Year>2014</Year><Month>Jul</Month></PubDate></JournalIssue><Title>FASEB journal : official publication of the Federation of American Societies for Experimental Biology</Title><ISOAbbreviation>FASEB J.</ISOAbbreviation></Journal><ArticleTitle>Estrogen-related receptor γ is an in vivo receptor of bisphenol A.</ArticleTitle><Pagination><MedlinePgn>3124-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1096/fj.13-240465</ELocationID><Abstract><AbstractText>Bisphenol A (BPA) is an endocrine disruptor that displays estrogenic activity. Several reports suggest that BPA may have estrogen receptor-independent effects. In zebrafish, 50 μM BPA exposure induces otic vesicle abnormalities, including otolith aggregation. The purpose of this study was to test if BPA action was mediated in vivo during zebrafish development by the orphan nuclear estrogen related receptor (ERR) γ. Combining pharmacological and functional approaches, we demonstrate that the zebrafish ERRγ mediates BPA-induced malformations in otoliths. Using different bisphenol derivatives, we show that different compounds can induce a similar otolith phenotype than BPA and that the binding affinity of these derivatives to the zebrafish ERRγ correlates with their ability to induce otolith malformations. Morpholino knockdown of ERRγ function suppresses the BPA effect on otoliths whereas overexpression of ERRγ led to a BPA-like otolith phenotype. Moreover, a subphenotypical dose of BPA (1 μM) combined with ERRγ overexpression led to a full-dose (50 μM) BPA otolith phenotype. We therefore conclude that ERRγ mediates the otic vesicle phenotype generated by BPA. Our results suggest that the range of pathways perturbed by this compound and its potential harmful effect are larger than expected.-Tohmé, M., Prud'homme, S. M., Boulahtouf, A., Samarut, E., Brunet, F., Bernard, L., Bourguet, W., Gibert, Y., Balaguer, P., Laudet, V. Estrogen-related receptor γ is an in vivo receptor of bisphenol A.</AbstractText><CopyrightInformation>© FASEB.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tohmé</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Prud'homme</LastName><ForeName>Sophie M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boulahtouf</LastName><ForeName>Abdel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Samarut</LastName><ForeName>Eric</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brunet</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>Laure</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourguet</LastName><ForeName>William</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>INSERM U1054, Centre de Biochimie Structurale, Montpellier, CNRS UMR5048, Universités Montpellier 1 and 2, Montpellier, France; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gibert</LastName><ForeName>Yann</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin School of Medicine, Waurn Ponds, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Balaguer</LastName><ForeName>Patrick</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Cancérologie de Montpellier, Institut National de la Santé et de la Recherche Médicale (INSERM) U896, Centre Régional de la Lutte Contre le Cancer Val d'Aurelle Paul Lamarque, Montpellier, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laudet</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 5242, Université Lyon 1, Université de Lyon, Lyon, France; vincent.laudet@ens-lyon.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>04</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>FASEB J</MedlineTA><NlmUniqueID>8804484</NlmUniqueID><ISSNLinking>0892-6638</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001559">Benzhydryl Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011960">Receptors, Estrogen</NameOfSubstance></Chemical><Chemical><RegistryNumber>MLT3645I99</RegistryNumber><NameOfSubstance UI="C006780">bisphenol A</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001559" MajorTopicYN="N">Benzhydryl Compounds</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010037" MajorTopicYN="N">Otolithic Membrane</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011960" MajorTopicYN="N">Receptors, Estrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015027" MajorTopicYN="N">Zebrafish</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">endocrine disrupters</Keyword><Keyword MajorTopicYN="N">otolith</Keyword><Keyword MajorTopicYN="N">zebrafish</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24744145</ArticleId><ArticleId IdType="pii">fj.13-240465</ArticleId><ArticleId IdType="doi">10.1096/fj.13-240465</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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